UniFrag and GenomePrimer: selection of primers for genome-wide production of unique amplicons

Sacha A F T van Hijum; Anne de Jong; Girbe Buist; Jan Kok; Oscar P Kuipers

doi:10.1093/bioinformatics/btg203

UniFrag and GenomePrimer: selection of primers for genome-wide production of unique amplicons

Bioinformatics. 2003 Aug 12;19(12):1580-2. doi: 10.1093/bioinformatics/btg203.

Authors

Sacha A F T van Hijum¹, Anne de Jong, Girbe Buist, Jan Kok, Oscar P Kuipers

Affiliation

¹ Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, PO Box 14, 9750 AA Haren, The Netherlands.

PMID: 12912842
DOI: 10.1093/bioinformatics/btg203

Abstract

The complementary programs UniFrag and GenomePrimer were developed to provide a reliable high-throughput method to select the most unique regions within genomic DNA sequence(s) and design primers therein, involving minimal user intervention and maximum flexibility.

Publication types

Evaluation Study

MeSH terms

Algorithms
DNA Primers / chemical synthesis
DNA Primers / chemistry*
DNA Primers / genetics*
Gene Expression Profiling / methods*
Oligonucleotide Array Sequence Analysis / instrumentation
Oligonucleotide Array Sequence Analysis / methods*
Sequence Alignment / methods*
Sequence Analysis, DNA / methods*
Software*

Substances

DNA Primers