[Stimulatory effect of chemokine-like factor 1 (CKLF1) on the growth of bone marrow cells]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Apr;23(2):119-22.
[Article in Chinese]

Abstract

Objective: To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells.

Methods: Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony formation of human hematopoietic progenitor cells was identified in semi-solid culture.

Results: CKLF1 has obvious enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF.

Conclusion: CKLF1 can promote the proliferation and differentiation of bone marrow cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / drug effects
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cells, Cultured
  • Chemokines / pharmacology*
  • Colony-Forming Units Assay
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / drug effects
  • Humans
  • MARVEL Domain-Containing Proteins
  • Mice
  • Mice, Inbred BALB C

Substances

  • CKLF protein, human
  • CKLF1 protein, mouse
  • Chemokines
  • MARVEL Domain-Containing Proteins