Synthesis of 2-5As possessing base-modified adenosines and their activities to human recombinant RNase L

Y Kitade; Y Tanida; T Tuboi; A Yoshimura; M Nakanishi; C Yatome

doi:10.1093/nass/44.1.29

Synthesis of 2-5As possessing base-modified adenosines and their activities to human recombinant RNase L

Nucleic Acids Symp Ser. 2000:(44):29-30. doi: 10.1093/nass/44.1.29.

Authors

Y Kitade¹, Y Tanida, T Tuboi, A Yoshimura, M Nakanishi, C Yatome

Affiliation

¹ Laboratory of Molecular Biochemistry, Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.

PMID: 12903252
DOI: 10.1093/nass/44.1.29

Abstract

The unique 2',5'-oligoadenylate (2-5A) acts as a potent inhibitor of translation in vertebrate cells through the activation of a constituent latent 2-5A-dependent endoribonuclease (RNase L). This 2-5A system plays a major role in the interferon natural defense mechanism against viral infection. We report the syntheses of base-modified adenosine-substituted 2-5A derivatives, their interaction with recombinant human RNase L and their biological stability.

MeSH terms

Adenine Nucleotides / chemical synthesis
Adenine Nucleotides / chemistry*
Adenine Nucleotides / metabolism*
Adenosine / analogs & derivatives*
Adenosine / chemical synthesis
Adenosine / chemistry
Adenosine / metabolism
Endoribonucleases / metabolism*
Humans
In Vitro Techniques
Kinetics
Molecular Structure
Oligoribonucleotides / chemical synthesis
Oligoribonucleotides / chemistry*
Oligoribonucleotides / metabolism*
Recombinant Proteins / metabolism
Substrate Specificity

Substances

Adenine Nucleotides
Oligoribonucleotides
Recombinant Proteins
2',5'-oligoadenylate
Endoribonucleases
2-5A-dependent ribonuclease
Adenosine