Tandem oligonucleotide synthesis using linker (First Base) phosphoramidites

Nucleic Acids Res Suppl. 2002:(2):1-2. doi: 10.1093/nass/2.1.1.

Abstract

First Base linker phosphoramidites add the first nucleoside to underivatized solid-phase supports using a regular phosphoramidite coupling cycle. A cleavable 3'-O-ester linkage allows NH4OH treatment to rapidly generate products with only 3'-OH ends. Either single or multiple sequences linked in tandem can be prepared and no additional deprotection steps are required.

MeSH terms

  • Amides / chemistry*
  • Electrophoresis, Capillary
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Phosphoric Acids / chemistry*

Substances

  • Amides
  • Oligonucleotides
  • Phosphoric Acids
  • phosphoramidic acid