Wild-type Escherichia coli producing microcins B17, D93, J25, and L; cloning of genes for microcin L production and immunity

Can J Microbiol. 2003 May;49(5):357-61. doi: 10.1139/w03-047.

Abstract

For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindIII-SalI fragment, which includes the Mcc V immunity gene, cvi.

MeSH terms

  • Bacteriocins / biosynthesis*
  • Bacteriocins / genetics*
  • Bacteriocins / immunology
  • Bacteriocins / pharmacology
  • Cloning, Molecular*
  • Drug Resistance, Bacterial
  • Escherichia coli / genetics*
  • Escherichia coli / immunology
  • Escherichia coli / isolation & purification
  • Escherichia coli / metabolism
  • Genes, Bacterial*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data

Substances

  • Bacteriocins
  • microcin

Associated data

  • GENBANK/AY237108