Abstract
In this communication, we show that the pacC(c)14 mutation drastically reduced the mannose and N-acetylglycosamine content of the pacA-encoded acid phosphatase secreted by the fungus Aspergillus nidulans when grown at 22 degrees C, pH 5.0, compared to a control strain. The staining after PAGE was not observed for the pacA-encoded acid phosphatase, while the palD-encoded Pi-repressible alkaline phosphatase had an altered electrophoretic mobility. In addition, the secreted acid phosphatase also had a reduced number of isoforms visualized by staining after IEF and glycosylation had a protective effect against its heat inactivation. We also show that a full-length version of gene pacC-1 cloned from Neurospora crassa complemented the pacC(c)14 mutation of A. nidulans, including the remediation of both the acid and alkaline Pi-repressible phosphatases secreted at pH 5.0, which indicates that glycosylation of secreted phosphatases is mediated in A. nidulans by the conserved PacC pathway that governs pH-responsive gene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase / biosynthesis
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Acid Phosphatase / chemistry
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Acid Phosphatase / isolation & purification
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Acid Phosphatase / metabolism
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Alkaline Phosphatase / biosynthesis
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Alkaline Phosphatase / metabolism
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Aspergillus nidulans / enzymology
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Aspergillus nidulans / genetics
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Aspergillus nidulans / metabolism*
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Chromatography
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Cloning, Molecular
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DNA, Fungal / isolation & purification
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Electrophoresis, Polyacrylamide Gel
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Fungal Proteins*
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Genes, Fungal
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Genes, Regulator*
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Genetic Complementation Test
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Glycosylation
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Hot Temperature
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Hydrogen-Ion Concentration
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Isoelectric Focusing
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Monosaccharides / analysis
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Mutation
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Neurospora crassa / genetics
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Phosphoric Monoester Hydrolases / chemistry
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Phosphoric Monoester Hydrolases / isolation & purification
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Phosphoric Monoester Hydrolases / metabolism*
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Staining and Labeling
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
Substances
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DNA, Fungal
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Fungal Proteins
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Monosaccharides
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PacC protein, Aspergillus
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Transcription Factors
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Alkaline Phosphatase
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Acid Phosphatase
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Phosphoric Monoester Hydrolases