cDNA cloning and mRNA expression of L-3,4-dihydroxyphenylalanine decarboxylase gene homologue from the silkworm, Bombyx mori

Biotechnol Lett. 2003 Jun;25(12):997-1002. doi: 10.1023/a:1024035424317.

Abstract

L-3,4-Dihydroxyphenylalanine decarboxylase (DDC) cDNA, from Bombyx mori that contains an open reading frame of 1437 bp encoding 478 amino acids, was cloned and characterized. Expression analyses of B. mori DDC mRNA by Northern and in situ hybridization indicated that expression of silkworm DDC expression is possibly controlled by neuropeptide hormones in tissue- and stage-specific manners.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bombyx / drug effects
  • Bombyx / enzymology*
  • Bombyx / genetics*
  • Carboxy-Lyases / biosynthesis*
  • Carboxy-Lyases / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Ecdysteroids / pharmacology
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Enzymologic / genetics*
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Analysis, DNA
  • Sequence Analysis, Protein

Substances

  • DNA, Complementary
  • Ecdysteroids
  • RNA, Messenger
  • Carboxy-Lyases
  • L-3,4-dihydroxyphenylalanine decarboxylase, Bombyx mori