Abstract
Recombinant tumstatin was expressed in stably transformed Drosophila melanogaster S2 cells and secreted into the medium with a molecular size of 29 kDa. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation. Purified recombinant tumstatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition for recombinant tumstatin was approx. 0.7 microg ml(-1). A maximum production of 4.6 microg recombinant tumstatin (10(7) cells)(-1) was obtained in a T-flask culture of S2 cells, 7 d after induction with 0.5 mM CuSO4.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoantigens / biosynthesis*
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Autoantigens / genetics*
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Autoantigens / isolation & purification
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Autoantigens / pharmacology
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Cattle
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Cell Division / drug effects
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Cell Division / physiology
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Collagen Type IV / biosynthesis*
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Collagen Type IV / genetics*
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Collagen Type IV / isolation & purification
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Collagen Type IV / pharmacology
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Drosophila melanogaster / classification
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / growth & development
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Drosophila melanogaster / metabolism*
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Gene Expression Regulation
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Humans
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Mutagenesis, Site-Directed
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Quality Control
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / pharmacology
Substances
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Autoantigens
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Collagen Type IV
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Recombinant Proteins
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type IV collagen alpha3 chain