Screening and characterization of astaxanthin-hyperproducing mutants of Haematococcus pluvialis

Biotechnol Lett. 2003 Apr;25(7):527-9. doi: 10.1023/a:1022877703008.

Abstract

Haematococcus pluvialis was mutated by UV or ethyl methanesulphonate. Mutants resistant to nicotine, diphenylamine, fluridone or norflurazon were then selected. Several nicotine-resistant mutants showed increased (1.9% to 2.5% vs. 1.2% w/w) astaxanthin production. Mutants maintained high astaxanthin production over 4 months of repeated culture.

MeSH terms

  • Biotechnology / methods*
  • Chlorophyta / drug effects
  • Chlorophyta / physiology*
  • Chlorophyta / radiation effects
  • Diphenylamine / pharmacology
  • Ethyl Methanesulfonate / pharmacology
  • Mutagenesis
  • Mutation*
  • Nicotine / pharmacology
  • Pyridazines / pharmacology
  • Pyridones / pharmacology
  • Ultraviolet Rays
  • Xanthophylls
  • beta Carotene / analogs & derivatives*
  • beta Carotene / biosynthesis*

Substances

  • Pyridazines
  • Pyridones
  • Xanthophylls
  • beta Carotene
  • fluridone
  • Nicotine
  • astaxanthine
  • Ethyl Methanesulfonate
  • Diphenylamine
  • norflurazone