Abstract
A plasmid pSDK-1 containing the Escherichia coli phosphofructokinase-1 gene (pfkA) was constructed, and transferred into Acidithiobacillus thiooxidans Tt-7 by conjugation. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium but the enzyme activity (18 U g-1) was lower than that in E. coli (K12: 86 U g-1; DF1010 carrying plasmid pSDK-1: 97 U g-1). In the presence of glucose, the Tt-7 transconjugant consumed glucose leading to a better growth yield.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Conjugation, Genetic
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Enzyme Activation
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Enzyme Stability
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Escherichia coli / classification
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Gammaproteobacteria / classification
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Gammaproteobacteria / genetics
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Gammaproteobacteria / growth & development*
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Gammaproteobacteria / metabolism*
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Gene Expression Regulation, Bacterial
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Gene Expression Regulation, Enzymologic
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Glucose / metabolism*
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Phosphofructokinase-1 / biosynthesis*
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Phosphofructokinase-1 / chemistry
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Phosphofructokinase-1 / genetics
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Protein Engineering / methods*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Species Specificity
Substances
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Recombinant Proteins
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Phosphofructokinase-1
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Glucose