Conversion of an obligate autotrophic bacteria to heterotrophic growth: expression of a heterogeneous phosphofructokinase gene in the chemolithotroph Acidithiobacillus thiooxidans

Biotechnol Lett. 2003 May;25(10):749-54. doi: 10.1023/a:1023588921918.

Abstract

A plasmid pSDK-1 containing the Escherichia coli phosphofructokinase-1 gene (pfkA) was constructed, and transferred into Acidithiobacillus thiooxidans Tt-7 by conjugation. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium but the enzyme activity (18 U g-1) was lower than that in E. coli (K12: 86 U g-1; DF1010 carrying plasmid pSDK-1: 97 U g-1). In the presence of glucose, the Tt-7 transconjugant consumed glucose leading to a better growth yield.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Conjugation, Genetic
  • Enzyme Activation
  • Enzyme Stability
  • Escherichia coli / classification
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Gammaproteobacteria / classification
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / growth & development*
  • Gammaproteobacteria / metabolism*
  • Gene Expression Regulation, Bacterial
  • Gene Expression Regulation, Enzymologic
  • Glucose / metabolism*
  • Phosphofructokinase-1 / biosynthesis*
  • Phosphofructokinase-1 / chemistry
  • Phosphofructokinase-1 / genetics
  • Protein Engineering / methods*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Species Specificity

Substances

  • Recombinant Proteins
  • Phosphofructokinase-1
  • Glucose