Effect of detergent on "promiscuous" inhibitors

J Med Chem. 2003 Jul 31;46(16):3448-51. doi: 10.1021/jm0340896.

Abstract

The term "promiscuous" inhibitors has been coined for compounds whose inhibition mechanism involves the interaction of aggregates of many compound molecules with the target protein, rather than the binding of individual molecules. This paper demonstrates that promiscuous inhibitors can be differentiated from classical 1:1 inhibitors by the judicious use of detergents, making it possible to configure assays that significantly reduce this undesirable mechanism of inhibition without compromising assay performance.

MeSH terms

  • Acetophenones / chemistry
  • Ampicillin / chemistry
  • Benzopyrans / chemistry
  • Boronic Acids / chemistry
  • Catalysis
  • Congo Red / chemistry
  • Detergents / chemistry*
  • Enterobacter cloacae / chemistry
  • Enzyme Inhibitors / chemistry*
  • Indoles / chemistry
  • Thiophenes / chemistry
  • beta-Lactamase Inhibitors*
  • beta-Lactamases / chemistry

Substances

  • 3-((2-thienyl)thiophenyliden-2-yl)-2H-indol-2-one
  • Acetophenones
  • Benzopyrans
  • Boronic Acids
  • Detergents
  • Enzyme Inhibitors
  • Indoles
  • Thiophenes
  • beta-Lactamase Inhibitors
  • thianaphthene-2-boronic acid
  • Congo Red
  • Ampicillin
  • rottlerin
  • beta-Lactamases