A great number of the reported examples of azo dye biodegradation comprise two main steps, the reductive cleavage of the azo bond under anaerobic conditions and the subsequent aerobic mineralization of the produced aromatic amines. Based on this possible metabolism a Sequencing Batch Reactor was chosen to study biologicalcolor removal from simulated cotton textile effluents containing a reactive azo dye. In previous studies high color removal levels of the azo dye Remazol Brilliant Violet 5R were achieved (up to 90% with an initial dye concentration of 100 mg l(-1)) during the anaerobic phase of Sequencing Batch Reactor operation. However, HPLC analyses revealed that the aromatic amines formed in the anaerobic phase were not mineralized during the subsequent aerobic phase. In an attempt to promote the aerobic biodegradation of these aromatic amines three different approaches were tested, the increase of the relative duration of the aerobic phase, the increase of the hydraulic retention time through the decrease of the daily fill flow and finally the increase of the dye/carbon source concentration ratio through the decrease of the fed volumetric organic load. The two aromatic amines directly resulting from azo bond reduction were detected by HPLC analysis. However, a third metabolite with significant peak area was also detected with a time profile suggesting an equilibrium with one of the aromatic amines In spite of the conversions occurring between metabolites during the cycles of the tested approaches, no effective biodegradation of these metabolites was observed during the experimental period of over 810 days.