The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine

Cell Res. 2003 Jun;13(3):195-202. doi: 10.1038/sj.cr.7290164.

Abstract

As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / analysis
  • Animals
  • Antigens, Surface / immunology
  • Biomarkers / analysis
  • Cell Culture Techniques / methods*
  • Cell Differentiation / physiology
  • DNA-Binding Proteins / immunology
  • Embryo, Mammalian / chemistry
  • Embryo, Mammalian / cytology*
  • Embryo, Mammalian / ultrastructure
  • Female
  • Germ Cells / chemistry
  • Germ Cells / cytology*
  • Germ Cells / ultrastructure
  • Immunohistochemistry
  • Karyotyping
  • Male
  • Microscopy, Electron
  • Octamer Transcription Factor-3
  • Stem Cells / chemistry
  • Stem Cells / cytology
  • Stem Cells / ultrastructure
  • Swine
  • Swine, Miniature
  • Transcription Factors*

Substances

  • Antigens, Surface
  • Biomarkers
  • DNA-Binding Proteins
  • Octamer Transcription Factor-3
  • Transcription Factors
  • Alkaline Phosphatase