We report here the two first cases of human ehrlichiosis described in Italy. One patient presented symptoms indicative of ehrlichiosis, such as fever, conjunctivitis, and increase of hepatic enzymes; HGE was confirmed by fourfold seroconversion for HGE antigen. The second patient, a forest worker, was affected by Lyme disease, and in this case HGE was suspected because of fever, leukopenia, and seroconversion.