Objective: To investigate the protecting effect on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through injury by transfection of recombinant adenovirus with whole long human heat shock protein 70 (HSP70) gene.
Methods: The recombinant adenoviruses were constructed with whole long human HSP70 gene. Cultured intestinal epithelial cells (IEC-6 cells) were divided into four groups: control group without transfection and 24 hours, 48 hours, 72 hours after transfection groups. The expression of human HSP70 gene in transfected cells were detected by reverse transcription-polymerase chain reaction (RT-PCR) after 48 hours transfection. Cells in different groups after 1 hour were treated with hypoxia followed by 1 hour of reoxygenation, then cell viability, growth curve and proliferative index were evaluated in vitro.
Results: The expression of human HSP70 gene in transfected IEC-6 cells was positive, while it was negative in control group. After treatment with hypoxia-reoxygenation, the cell viability in transfected groups was significantly higher than that of control (P<0.01), the proliferative index was also improved (P<0.05).
Conclusion: The proliferation ability of intestinal epithelial cells (IEC-6 cells) could be promoted by HSP70 gene transfection, which therefore could protect the IEC-6 cells against hypoxia-reoxygenation injury.