Abstract
Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcholinesterase
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Biosensing Techniques / instrumentation
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Biosensing Techniques / methods*
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Catechols / analysis
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Cholinesterases
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Electrochemistry / instrumentation
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Electrochemistry / methods*
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Electrodes
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Enzymes, Immobilized
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Flow Injection Analysis
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Horseradish Peroxidase
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Hydrogen Peroxide
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Monophenol Monooxygenase
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Multienzyme Complexes / chemistry*
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Phenols / analysis
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Reproducibility of Results
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Water Pollutants, Chemical / analysis
Substances
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Catechols
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Enzymes, Immobilized
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Multienzyme Complexes
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Phenols
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Water Pollutants, Chemical
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Hydrogen Peroxide
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Horseradish Peroxidase
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Monophenol Monooxygenase
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Acetylcholinesterase
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Cholinesterases