Characterization of the human HOX 7 cDNA and identification of polymorphic markers

Hum Mol Genet. 1992 Sep;1(6):407-10. doi: 10.1093/hmg/1.6.407.

Abstract

cDNA clones for a human HOX 7 gene obtained with homologous clones of Drosophila were used in human gene mapping studies. The human cDNA clone was isolated from a library constructed from human embryonic craniofacial material. The sequence of the cDNA demonstrates significant homology with mouse HOX 7. A search for RFLPs identified MboII and BstEII variants. A CA dinucleotide repeat with 5 alleles was also identified and allowed placement of HOX 7 into a defined linkage map. Evidence for linkage disequilibrium was found with markers tested. These results place the human HOX 7 gene in a defined position on 4p.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping / methods
  • Chromosomes, Human, Pair 4*
  • Cloning, Molecular / methods
  • DNA / genetics*
  • DNA / isolation & purification
  • Deoxyribonucleases, Type II Site-Specific
  • Drosophila / genetics
  • Embryo, Mammalian
  • Embryo, Nonmammalian
  • Female
  • Gene Library
  • Genes, Homeobox*
  • Genetic Variation
  • Humans
  • Linkage Disequilibrium
  • Male
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Pedigree
  • Polymorphism, Restriction Fragment Length*
  • RNA / genetics
  • RNA / isolation & purification
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Oligodeoxyribonucleotides
  • RNA
  • DNA
  • endodeoxyribonuclease MboII
  • Deoxyribonucleases, Type II Site-Specific
  • GGTNACC-specific type II deoxyribonucleases

Associated data

  • GENBANK/M88632
  • GENBANK/M88633
  • GENBANK/M88634
  • GENBANK/M88635
  • GENBANK/M88636
  • GENBANK/M88637
  • GENBANK/M97676
  • GENBANK/X64597
  • GENBANK/X64598
  • GENBANK/X64599