Abstract
The viral load assays AMPLICOR HIV-1 Monitor Test 1.5, Nuclisens HIV-1 QT, and Quantiplex HIV RNA 3.0 (bDNA) were evaluated for their abilities to quantify human immunodeficiency virus type 1 (HIV-1) RNA in 64 plasma samples from 21 children infected in Portugal. The children were infected with HIV-1 subtypes A1, B, F1, G, and BG recombinant virus. AMPLICOR v1.5 and Quantiplex v3.0 detected all samples, and there was a good correlation of results between the two kits. Thirty-eight specimens containing HIV-1 subtype B, G, or recombinant BG, could not be detected by Nuclisens HIV-1 QT. We also evaluated the new Retina HIV-1 assay on 21 samples that were HIV-1 positive; Retina HIV-1 failed to detect 5 of 11 subtype G specimens. AMPLICOR v1.5 and Quantiplex v3.0 assays may be used for HIV-1 RNA quantification in Portugal, whereas an improvement in sensitivity for subtype G and recombinant BG is required for Nuclisens HIV-1 QT and Retina HIV-1.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Branched DNA Signal Amplification Assay
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Child
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Child, Preschool
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Female
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HIV Infections / virology*
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HIV-1 / classification*
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HIV-1 / genetics
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Humans
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Male
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Molecular Sequence Data
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RNA, Viral / blood*
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Reagent Kits, Diagnostic*
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Recombination, Genetic
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Viral Load*
Substances
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RNA, Viral
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Reagent Kits, Diagnostic
Associated data
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GENBANK/AY161864
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GENBANK/AY161865
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GENBANK/AY161866
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GENBANK/AY161867
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GENBANK/AY161868
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GENBANK/AY161869
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GENBANK/AY161870
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GENBANK/AY161884
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GENBANK/AY161885
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GENBANK/AY161886
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GENBANK/AY161894