Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections

J Clin Microbiol. 2003 Jul;41(7):3351-3. doi: 10.1128/JCM.41.7.3351-3353.2003.

Abstract

To further evaluate recombinant Em18 antigen (rEm18) for immunodiagnosis of human alveolar echinococcosis, 208 serum samples were examined by enzyme-linked immunosorbent assay (ELISA). To comparatively assess the results of rEm18-ELISA, ELISA and immunoblot analysis with two affinity-purified native antigens were also performed with 45 selected serum samples. The results indicate that rEm18 is highly useful for serodiagnosis.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Helminth / blood*
  • Antigens, Helminth / genetics
  • Antigens, Helminth / immunology*
  • Chromatography, Affinity
  • Echinococcosis / diagnosis
  • Echinococcosis / parasitology*
  • Echinococcosis, Hepatic / diagnosis
  • Echinococcosis, Hepatic / parasitology
  • Echinococcus / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoblotting
  • Recombinant Proteins / immunology

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Em18 antigen
  • Recombinant Proteins