[The expression and activity of caspase-8 in the process of vitamin E succinate-induced apoptosis in human gastric carcinoma SGC-7901 cells]

Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Mar;37(2):112-4.
[Article in Chinese]

Abstract

Objective: To investigate the role of caspase-8 in vitamin E succinate (VES)-induced apoptosis in human gastric carcinoma cells.

Methods: The expression and activity of caspase-8 were measured by the Western Blot method with fluorescence and DAPI staining.

Results: VES treatment induced the expression and activity of caspase-8 in a dose-dependent manner; the apoptosis ratio induced by VES was significantly decreased in SGC-7901 cells treated with caspase-8 inhibitor.

Conclusion: Caspase-8 plays an important role in SGC-7901 cell apoptosis induced by VES.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Caspase 8
  • Caspases / metabolism*
  • Caspases / physiology
  • Dose-Response Relationship, Drug
  • Humans
  • Stomach Neoplasms / enzymology
  • Stomach Neoplasms / pathology*
  • Tocopherols
  • Tumor Cells, Cultured
  • Vitamin E / analogs & derivatives*
  • Vitamin E / pharmacology*

Substances

  • Vitamin E
  • CASP8 protein, human
  • Caspase 8
  • Caspases
  • Tocopherols