Abstract
The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) has created a unique approach to protein investigations. Surface plasmon resonance is used to quantify interactions between proteins and surface-immobilized ligands, and MS is used to determine the structural features of the bound proteins. Recent progress in SPR-MS includes improved methods and operations, increased limits of detection, multi-protein analysis and protein-complex delineation. With the subsequent design of SPR protein arrays, SPR-MS is expected to enter into the field of high-throughput protein interaction discovery and miniaturized diagnostics.
Publication types
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Mass Spectrometry / instrumentation
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Mass Spectrometry / methods*
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Mass Spectrometry / trends*
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Microchemistry / instrumentation
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Microchemistry / methods
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Microchemistry / trends
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Molecular Weight
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Nanotechnology / instrumentation
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Nanotechnology / methods
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Nanotechnology / trends
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Protein Array Analysis / instrumentation
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Protein Array Analysis / methods*
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Protein Array Analysis / trends*
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Protein Binding
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Protein Conformation
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Proteins / chemistry*
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Proteins / classification
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Surface Plasmon Resonance / instrumentation
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Surface Plasmon Resonance / methods*
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Surface Plasmon Resonance / trends*
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Systems Integration