Study on structure of ribosomal RNA by time-resolved luminescence anisotropy analysis

T Sakamoto; A Mahara; R Iwase; T Yamaoka; A Murakami

doi:10.1093/nass/1.1.143

Study on structure of ribosomal RNA by time-resolved luminescence anisotropy analysis

Nucleic Acids Res Suppl. 2001:(1):143-4. doi: 10.1093/nass/1.1.143.

Authors

T Sakamoto¹, A Mahara, R Iwase, T Yamaoka, A Murakami

Affiliation

¹ Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.

PMID: 12836305
DOI: 10.1093/nass/1.1.143

Abstract

Evaluation of the folded structure of E.coli 16S-rRNA was performed using 5'-Ru(II) complex labeled oligodeoxyribonucleotide (Ru-probe) as the probe. This probe could evaluate the dynamic manner of the binding site of Ru-probe by using the time-resolved luminescence anisotropy technique. It was found that the rotational manner of Ru-probe in the presence of 16S-rRNA was markedly dependent on the oligonucleotide sequence, indicating that the microenvironments around the Ru-probes were largely different.

MeSH terms

Anisotropy
Base Sequence
Binding Sites
Escherichia coli / genetics
Luminescent Measurements
Oligodeoxyribonucleotides / chemistry*
Oligodeoxyribonucleotides / metabolism
Organometallic Compounds / chemistry*
Organometallic Compounds / metabolism
Phenanthrolines / chemistry*
Phenanthrolines / metabolism
RNA, Ribosomal, 16S / chemistry*
RNA, Ribosomal, 16S / metabolism
Rotation
Time Factors

Substances

Oligodeoxyribonucleotides
Organometallic Compounds
Phenanthrolines
RNA, Ribosomal, 16S
tris-(1,10-phenanthroline)ruthenium