Objective: To probe the mutations of RB gene in laryngeal carcinoma, and the relation between it and the clinical phase.
Method: The mutations were detected by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism).
Result: In laryngeal carcinoma, the mutations were identified in 14(15.5%) of 90 samples, of which 2 were large deletion, 12 were abnormal in electrophoresis of SSCP. The mutations of I-II stage tumor were 5(13.9%), of III-IV were 9(16.7%), there wasn't a significant difference between them.
Conclusion: RB gene mutation takes part in the development of laryngeal carcinoma. But there wasn't relation between RB gene mutation and the course of laryngeal carcinoma.