Abstract
Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transcriptional activator involved in the immortalization of B lymphocytes by the virus. EBNA2 is targeted to the promoters of its responsive genes, via interaction with cellular DNA-binding proteins. Using chromatin immunoprecipitation assays, we show for the first time the conditional recruitment of EBNA2 on two specific viral promoters in vivo and demonstrate a correlation between this recruitment and a local change in the acetylation of histones H3 and H4, which is promoter dependent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Cell Line
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Cell Transformation, Viral
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Chromatin / metabolism*
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Epstein-Barr Virus Nuclear Antigens / genetics
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Epstein-Barr Virus Nuclear Antigens / metabolism*
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Gene Expression Regulation, Viral
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / physiology*
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Histones / metabolism*
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Humans
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Precipitin Tests
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Promoter Regions, Genetic*
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Viral Proteins
Substances
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Chromatin
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EBNA-2 protein, Human herpesvirus 4
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Epstein-Barr Virus Nuclear Antigens
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Histones
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Viral Proteins