A polymorphic, telomeric-like sequence microsatellite in the Neotropical fish Prochilodus

T Hatanaka; F Henrique-Silva; P M Galetti Jr

doi:10.1159/000071054

A polymorphic, telomeric-like sequence microsatellite in the Neotropical fish Prochilodus

Cytogenet Genome Res. 2002;98(4):308-10. doi: 10.1159/000071054.

Authors

T Hatanaka¹, F Henrique-Silva, P M Galetti Jr

Affiliation

¹ Departamento de Genética e Evolução, Universidade Federal de São Carlos, Rodovia Washington Luiz Km 235, CEP 13565-905 São Carlos, SP, Brazil.

PMID: 12826759
DOI: 10.1159/000071054

Abstract

A microsatellite locus from the Neotropical fish genus Prochilodus was isolated using PCR-based isolation of microsatellite arrays. Of 470 positive clones, 15 were sequenced, and 10 of them showed an (AATTT)(n) repeat. Primers were designed, and analysis of polymorphism revealed 11 alleles in three Prochilodus species. Fluorescence in situ hybridization analysis showed signals predominantly in the telomeric regions of several chromosomes. The description of this microsatellite may contribute to studies of the population structure of this fish group.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fishes / genetics*
In Situ Hybridization, Fluorescence
Microsatellite Repeats / genetics*
Species Specificity
Telomere / genetics*
Tropical Climate