The "Istituti Zooprofilattici" are an important network whose main function is the monitoring of animal health as well as food. As a result of the recent improvements in aquaculture technology interest in the safety of seafood is increasing. Therefore, the purpose of this study was to set up diagnostic methods for the detection of virus contamination, as well as the use of in vitro techniques able to identify the different toxins. The results have allowed the development of molecular biology assays which, together with the isolation in cell cultures, can detect contaminations/infections by the hepatitis A virus and the most common enteroviruses. Moreover, specific selected cell lines have led to the detection of different toxins. These laboratory methods will be used in order to control seafood safety.