Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB

Biogerontology. 2003;4(3):125-31. doi: 10.1023/a:1024154024602.

Abstract

In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H(2)O(2)-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival / drug effects
  • Cell Survival / radiation effects
  • Cells, Cultured
  • Cellular Senescence / drug effects
  • Diploidy
  • Ethanol / pharmacology
  • Fibroblasts / cytology*
  • Fibroblasts / physiology*
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Viral
  • Humans
  • Hydrogen Peroxide / pharmacology
  • Oxidants / pharmacology
  • Oxidative Stress / drug effects
  • Peroxidases / genetics*
  • Peroxiredoxin VI
  • Peroxiredoxins
  • Retroviridae / genetics*
  • Solvents / pharmacology
  • Transfection
  • Ultraviolet Rays
  • tert-Butylhydroperoxide / pharmacology*

Substances

  • Oxidants
  • Solvents
  • Ethanol
  • tert-Butylhydroperoxide
  • Hydrogen Peroxide
  • Peroxidases
  • Peroxiredoxin VI
  • Peroxiredoxins