Abstract
In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H(2)O(2)-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Survival / drug effects
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Cell Survival / radiation effects
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Cells, Cultured
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Cellular Senescence / drug effects
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Diploidy
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Ethanol / pharmacology
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Fibroblasts / cytology*
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Fibroblasts / physiology*
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Viral
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Humans
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Hydrogen Peroxide / pharmacology
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Oxidants / pharmacology
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Oxidative Stress / drug effects
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Peroxidases / genetics*
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Peroxiredoxin VI
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Peroxiredoxins
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Retroviridae / genetics*
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Solvents / pharmacology
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Transfection
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Ultraviolet Rays
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tert-Butylhydroperoxide / pharmacology*
Substances
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Oxidants
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Solvents
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Ethanol
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tert-Butylhydroperoxide
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Hydrogen Peroxide
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Peroxidases
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Peroxiredoxin VI
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Peroxiredoxins