NMR experiments for the measurement of proton-proton and carbon-carbon residual dipolar couplings in uniformly labelled oligosaccharides

Manuel Martin-Pastor; Angeles Canales-Mayordomo; Jesús Jiménez-Barbero

doi:10.1023/a:1024096807537

NMR experiments for the measurement of proton-proton and carbon-carbon residual dipolar couplings in uniformly labelled oligosaccharides

J Biomol NMR. 2003 Aug;26(4):345-53. doi: 10.1023/a:1024096807537.

Authors

Manuel Martin-Pastor¹, Angeles Canales-Mayordomo, Jesús Jiménez-Barbero

Affiliation

¹ Laboratorio Integral de Estructura de Biomoléculas Jose. R. Carracido, Unidade de Resonancia Magnética, RIAIDT, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. mmartin@usc.es

PMID: 12815261
DOI: 10.1023/a:1024096807537

Abstract

A 2D-HSQC-carbon selective/proton selective-constant time COSY, 2D-HSQC-(sel C, sel H)-CT COSY experiment, which is applicable to uniformly (13)C isotopically enriched samples (U-(13)C) of oligosaccharides or oligonucleotides is proposed for the measurement of proton-proton RDC in crowded regions of 2D-spectra. In addition, a heteronuclear constant time-COSY experiment, (13)C-(13)C CT-COSY, is proposed for the measurement of one bond carbon-carbon RDC in these molecules. These two methods provide an extension, to U-(13)C molecules, of the original homonuclear constant time-COSY experiment proposed by Tian et al. (1999) for saccharides. The combination of a number of these RDC with NOE data may provide the method of choice to study oligosaccharide conformation in the free and receptor-bound state.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation*
Carbon / chemistry*
Nuclear Magnetic Resonance, Biomolecular / methods*
Oligosaccharides / chemistry*
Protons*

Substances

Oligosaccharides
Protons
Carbon