Abstract
Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound-degrading bacteria in soil samples by using the FISH method. Also, amplification by PCR using a set of ERIC primers was assayed for the detection of Pseudomonas GCH1 strain, which used in the soil bioremediation process. A model was developed to understand and predict the behavior of bacteria and pollutants in a bioremediation system, taking into account fluid dynamics, molecular/cellular scale processes, and biofilm formation.
Publication types
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Alginates / chemistry
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Biodegradation, Environmental*
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Biofilms / growth & development*
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DNA Probes / genetics
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DNA, Bacterial / genetics
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Dioxygenases*
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Glucuronic Acid / chemistry
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Gram-Positive Bacteria / enzymology
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Gram-Positive Bacteria / genetics
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Gram-Positive Bacteria / metabolism*
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Hexuronic Acids / chemistry
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Hydrocarbons, Aromatic / analysis
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Hydrocarbons, Aromatic / metabolism
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In Situ Hybridization, Fluorescence
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Models, Biological
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Oligonucleotides / analysis
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Oligonucleotides / genetics
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Oxygenases / analysis
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Oxygenases / genetics
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Oxygenases / metabolism
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Polymerase Chain Reaction / methods
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Predictive Value of Tests
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / growth & development*
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Research Design
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Soil Microbiology*
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Water Microbiology*
Substances
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Alginates
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DNA Probes
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DNA, Bacterial
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Hexuronic Acids
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Hydrocarbons, Aromatic
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Oligonucleotides
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Glucuronic Acid
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Oxygenases
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Dioxygenases
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3,4-dihydroxyphenylacetate 2,3-dioxygenase