Naturally contaminated corn samples of different origin were extracted using two conventional techniques (blending and shaking) and three alternative approaches (ultrasonic extraction, accelerated solvent extraction, and microwave-assisted extraction). Use of the same extraction mixture for all trials enabled the efficiency of the various extraction techniques to be compared. Extracts were filtered and directly analyzed by LC-ESI-MS, without further clean-up. The yield from the alternative extraction techniques showed efficiency to be higher than for conventional techniques. In particular, microwave-assisted extraction was slightly superior to other techniques.