Capturing ion exchanger-bound infectious pancreatic necrosis virus--design and application for large volume water samples

J Virol Methods. 2003 Jun 30;110(2):173-8. doi: 10.1016/s0166-0934(03)00126-5.

Abstract

A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 l water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger. Then the resin was collected rapidly and quantitatively through a specific device which we designed. The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide gel electrophoresis and Western blot analysis. Serological results were obtained within 24 h after the water sample was introduced into the laboratory. Proteins were recovered at an efficiency between 70 and 80% and the total concentration factor ranged between 150000 and 250000 times, depending on the requirements and the methods of choice.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Animals
  • Cells, Cultured
  • Chromatography, Ion Exchange / instrumentation*
  • Chromatography, Ion Exchange / methods*
  • Fibroblasts
  • Filtration / instrumentation
  • Infectious pancreatic necrosis virus / classification
  • Infectious pancreatic necrosis virus / isolation & purification*
  • Infectious pancreatic necrosis virus / pathogenicity
  • Ion Exchange Resins
  • Salmon
  • Virology / methods
  • Water Microbiology*

Substances

  • Ion Exchange Resins