Determination of inorganic phosphate in serum and saliva using a synthetic receptor

Org Lett. 2003 Jun 12;5(12):2029-31. doi: 10.1021/ol034427x.

Abstract

[structure: see text] A C(3)(v) symmetric synthetic receptor (1) was employed in an indicator-displacement assay to determine the phosphate concentrations in both horse serum and human saliva at biological pH. The determination of the phosphate concentrations in the serum and saliva using the colorimetric assay were 1.6 and 5.1 mM, respectively. These results further accentuate the usefulness of synthetic receptors in truly practical applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Colorimetry / methods
  • Copper / chemistry*
  • Fluoresceins / chemistry
  • Horses
  • Humans
  • Kinetics
  • Organometallic Compounds / chemistry*
  • Phosphates / analysis*
  • Phosphates / blood*
  • Saliva / chemistry*
  • Spectrophotometry, Ultraviolet

Substances

  • Fluoresceins
  • Organometallic Compounds
  • Phosphates
  • 6-carboxyfluorescein
  • Copper