Objective: To provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa.
Method: Nineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers.
Result: The 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers, which divided the 19 varieties into four groups: Group Beijing, Group 85-5, Group Guolimao and the other Group.
Conclusion: 8 varieties of Group Beijing have a close genetic relationship, and so have varieties of Group 85-5, which provides information for Rehmannia glutinosa's breeding.