[Induction of callus of Hypericum perforatum L. and qualitative identification of active constituents of its callus]

Zhongguo Zhong Yao Za Zhi. 2001 Dec;26(12):813-5.
[Article in Chinese]

Abstract

Objective: To induce the callus of H. perforatum and identify hypericin and pseudohypericin of its callus.

Method: The callus was induced in different culture conditions, and active constituents were determined by HPLC.

Result and conclusion: The inductions of callus from different parts were discussed, the induction rate of the leaf axil being the highest. The MS basic medium with 4 micrograms.L-1 2,4-D and 0.2 microgram.L-1 6-BA was the best of all screened media. Hypericin in the callus is determined by HPLC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthracenes
  • Culture Media
  • Culture Techniques / methods
  • Hypericum / chemistry
  • Hypericum / growth & development*
  • Perylene / analogs & derivatives*
  • Perylene / analysis*
  • Plant Leaves / chemistry
  • Plants, Medicinal / chemistry*
  • Plants, Medicinal / growth & development*

Substances

  • Anthracenes
  • Culture Media
  • Perylene
  • hypericin
  • pseudohypericin