Objective: A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.
Method: The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm.
Result: The standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively.
Conclusion: It provides scientific indexes for quality control of T. foenum-graecum.