[Determination of gossypol in cotton root bark by HPLC]

Zhongguo Zhong Yao Za Zhi. 2002 Mar;27(3):173-5.
[Article in Chinese]

Abstract

Objective: To establish a method for the determination of gossypol in cotton root bark.

Method: Samples were extracted with acetone, and determined on a C18 column with the mobile phase (Acetonitrile-0.2% phosphoric acid, 85:15) and UV-235 nm detector.

Result: The recovery rate was 97.6%, RSD 1.59% (n = 5).

Conclusion: This method can be used for the determination of gossypol in cotton root bark and the content of gossypol in three different species has been determined.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Gossypium / chemistry*
  • Gossypol / analysis*
  • Plant Roots / chemistry
  • Plants, Medicinal / chemistry*

Substances

  • Gossypol