Objective: To detect the mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs.
Methods: After consulting gene bank, we analyzed conservative sequence of hyaluronan synthases in different species, detected the mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs with oligonucleotide probe by hybridization in situ.
Results: mRNA of hyaluronan synthase was strongly expressed in some epithelial cells of endolymphatic sac, coupled with negative expression in negative control groups.
Conclusion: It confirms that endolymphatic sac cells can synthesize hyaluronan.