Promoter of the rolC gene of Agrobacterium rhizogenes can be strongly regulated in glandular cell of transgenic tobacco

Mol Biotechnol. 2003 Jun;24(2):121-6. doi: 10.1385/MB:24:2:121.

Abstract

A 577-bp promoter segment of Agrobacterium rhizogenes rolC, previously known as the phloem-specific gene expression promoter, was fused to the 5' end of a reporter gene, beta-glucuronidase (GUS), uidA. This rolC-promoter-driven expression of the GUS gene was found to be significantly strong in glandular cells in transgenic tobacco plants. Analysis of this segment of the promoter sequence revealed a myb response element.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Enzyme Activation
  • Gene Expression Regulation, Plant / genetics*
  • Genetic Engineering / methods*
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Molecular Sequence Data
  • Nicotiana / enzymology*
  • Nicotiana / genetics*
  • Plant Leaves / enzymology
  • Plant Leaves / genetics
  • Plant Roots / enzymology
  • Plant Roots / genetics
  • Plants, Genetically Modified / enzymology
  • Plants, Genetically Modified / genetics
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Rhizobium / enzymology*
  • Rhizobium / genetics*
  • beta-Glucosidase / genetics*
  • beta-Glucosidase / metabolism*

Substances

  • Recombinant Fusion Proteins
  • cytokinin-beta-glucosidase
  • beta-Glucosidase
  • Glucuronidase