Abstract
A 577-bp promoter segment of Agrobacterium rhizogenes rolC, previously known as the phloem-specific gene expression promoter, was fused to the 5' end of a reporter gene, beta-glucuronidase (GUS), uidA. This rolC-promoter-driven expression of the GUS gene was found to be significantly strong in glandular cells in transgenic tobacco plants. Analysis of this segment of the promoter sequence revealed a myb response element.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Enzyme Activation
-
Gene Expression Regulation, Plant / genetics*
-
Genetic Engineering / methods*
-
Glucuronidase / genetics
-
Glucuronidase / metabolism
-
Molecular Sequence Data
-
Nicotiana / enzymology*
-
Nicotiana / genetics*
-
Plant Leaves / enzymology
-
Plant Leaves / genetics
-
Plant Roots / enzymology
-
Plant Roots / genetics
-
Plants, Genetically Modified / enzymology
-
Plants, Genetically Modified / genetics
-
Promoter Regions, Genetic
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Rhizobium / enzymology*
-
Rhizobium / genetics*
-
beta-Glucosidase / genetics*
-
beta-Glucosidase / metabolism*
Substances
-
Recombinant Fusion Proteins
-
cytokinin-beta-glucosidase
-
beta-Glucosidase
-
Glucuronidase