Cloning of the gene coding for Staphylococcus intermedius exfoliative toxin and its expression in Escherichia coli

Vet Microbiol. 2003 Jun 24;94(1):31-8. doi: 10.1016/s0378-1135(03)00047-6.

Abstract

An exfoliative toxin (SIET)-producing strain (D-52) of Staphylococcus intermedius derived from canine pyoderma did not possess large plasmids. Therefore, the gene coding for SIET was considered to be located on the chromosomal DNA. The SIET gene was cloned from the chromosomal DNA of S. intermedius and was expressed in Escherichia coli. The nucleotide sequence of the SIET gene consists of a coding region of 990 bp specifying a polypeptide of 330 amino acid residues, which included a putative 42-residue signal sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Toxins / biosynthesis
  • Bacterial Toxins / genetics*
  • Base Sequence
  • Chickens
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Exfoliatins / biosynthesis
  • Exfoliatins / genetics*
  • Genes, Bacterial / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Sequence Homology, Amino Acid
  • Staphylococcus / genetics*
  • Staphylococcus / metabolism

Substances

  • Bacterial Toxins
  • DNA, Bacterial
  • Exfoliatins
  • Recombinant Proteins