Various physiological parameters of Lentinula edodes (Shiitake) in the presence of nine heavy metal salts were investigated. The mycelial growth was highly sensitive to cadmium and mercury, but less sensitive to zinc, copper, and lead. This resistance can be particularly dangerous to humans in the case of edible fungi such as Shiitake because of the possible heavy metal accumulation during growth and fruiting body production. All of the tested heavy metals inhibited decolorization of the dye Poly R-478 and the production of manganese peroxidase to a greater extent than they inhibited growth. Interestingly, with the exception of iron, the addition of all heavy metal salts investigated led to the increase of laccase production. Apart from cadmium and iron, none of the heavy metals inhibited the in vitro enzyme activities in concentrations up to 3mM. The results of this study indicated the applicability of L. edodes in biosorption technologies used in the removal of toxic metals from contaminated effluents and in bioremediation technologies designed to treat complex wastes contaminated with heavy metals in addition to other xenobiotics.