Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis

J Clin Microbiol. 2003 May;41(5):2174-5. doi: 10.1128/JCM.41.5.2174-2175.2003.

Abstract

We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chlamydia Infections / diagnosis*
  • Chlamydia Infections / microbiology
  • Chlamydia Infections / transmission
  • Chlamydia trachomatis* / genetics
  • Homosexuality, Male
  • Humans
  • Ligase Chain Reaction / methods*
  • Male
  • Pilot Projects
  • Polymerase Chain Reaction / methods*
  • Rectal Diseases / diagnosis*
  • Rectal Diseases / microbiology