Patients with uremia show a tendency for hemorrhagic diathesis and thrombi formation. These disorders have been attributed to functional abnormalities of platelet and their interaction with the vascular wall. The aim of the present study was to elucidate whether and how in patients with uremia, hemodialysis affects the level of platelet activation markers, i.e.: beta-TG and PF4, and whether the changes in the examined parameters depend on creatinine level and on the type dialysis membranes used during HD. The study involved 70 patients with uremia subjected to repeated hemodialysis and 70 healthy subjects. Prior to HD, we observed a decrease in PLT, and increase in beta-TG concentration and a drop in PF4 (p = 0.000), compared to control group. Immediately after the procedure a significant increase was observed in PLT and decrease in PF4 concentration, as well as a slight rise in beta-TG level, compared to the values obtained before HD. We also found statistically significant differences in beta-TG and PF4 concentrations prior to HD, irrespective of creatinine concentration, compared to control group. Only at creatinine concentration of 10.0-15.5 mg/dL, PLT differences significantly from PLT in control group. Following HD, creatinine-dependent statistically significant changes were noted in PLT, in comparison to the values obtained before the procedure. The analysis of platelet parameters revealed no statistically significant differences associated with the type of the dialysis membrane used for HD.