Abstract
Real-time PCR assays were established with the Toxoplasma gondii P30 and the 35-copy B1 gene as target genes. Both PCR methods detected Toxoplasma DNA from 10 to 100000 genome equivalents per assay and had comparable intra-assay deviations suggesting that detecting a single copy locus is suitable for clinical diagnostic.
MeSH terms
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Adolescent
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Adult
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Animals
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Antigens, Protozoan*
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Base Sequence
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Female
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Genes, Protozoan / genetics*
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Genes, erbB-1 / genetics*
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Humans
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Male
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Protozoan Proteins / genetics*
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Sensitivity and Specificity
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Toxoplasma / genetics*
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Toxoplasma / isolation & purification
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Toxoplasmosis / diagnosis
Substances
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Antigens, Protozoan
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Protozoan Proteins
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SAG1 antigen, Toxoplasma