Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii

Diagn Microbiol Infect Dis. 2003 Apr;45(4):269-71. doi: 10.1016/s0732-8893(02)00549-7.

Abstract

Real-time PCR assays were established with the Toxoplasma gondii P30 and the 35-copy B1 gene as target genes. Both PCR methods detected Toxoplasma DNA from 10 to 100000 genome equivalents per assay and had comparable intra-assay deviations suggesting that detecting a single copy locus is suitable for clinical diagnostic.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Antigens, Protozoan*
  • Base Sequence
  • Female
  • Genes, Protozoan / genetics*
  • Genes, erbB-1 / genetics*
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Protozoan Proteins / genetics*
  • Sensitivity and Specificity
  • Toxoplasma / genetics*
  • Toxoplasma / isolation & purification
  • Toxoplasmosis / diagnosis

Substances

  • Antigens, Protozoan
  • Protozoan Proteins
  • SAG1 antigen, Toxoplasma