Abstract
Seven antioxidative compounds were isolated from the EtOAc extract of the aerial part of C. sessiliflora (Japanese name, tanukimame) by activity-guided fractionation with 2,2-diphenyl-1-picrylhydrazyl (DPPH). Among the isolated compounds, hydroxyeucomic acid showed the strongest free radical-scavenging activity, which was almost identical to that of epigallocatechin gallate, against DPPH. Orientin and isoorientin showed strong anti-peroxidative activities toward linoleic acid and protective effects against the bactericidal action of the tert-butyl peroxyl radical. Their activities were nearly equal to that of epigallocatechin gallate.
MeSH terms
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Antioxidants / chemistry*
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Biphenyl Compounds / chemistry
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Catechin / analogs & derivatives*
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Catechin / pharmacology
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Chemical Fractionation / methods
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Crotalaria / chemistry*
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Free Radical Scavengers / analysis
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Free Radical Scavengers / pharmacology
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Free Radicals / antagonists & inhibitors
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Hydrazines / chemistry
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Linoleic Acid / metabolism
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Lipid Peroxidation / drug effects
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Oxidation-Reduction
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Picrates
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Plant Components, Aerial / chemistry
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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tert-Butylhydroperoxide / chemistry
Substances
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Antioxidants
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Biphenyl Compounds
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Free Radical Scavengers
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Free Radicals
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Hydrazines
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Picrates
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Plant Extracts
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Catechin
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tert-Butylhydroperoxide
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Linoleic Acid
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epigallocatechin gallate
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1,1-diphenyl-2-picrylhydrazyl