Selective internalization of monoclonal antibodies by B-cell chronic lymphocytic leukaemia cells

Br J Haematol. 2003 May;121(3):458-61. doi: 10.1046/j.1365-2141.2003.04305.x.

Abstract

B-cell chronic lymphocytic leukaemia (B-CLL) cannot be cured by conventional chemotherapy, therefore, toxin-linked therapeutic monoclonal antibodies (mAbs) are increasingly examined for their potential to improve clinical outcome. The current study aimed to identify mAbs that were internalized by the B-CLL cells of 14 patients, using both flow cytometry and confocal laser scanning microscopy. Anti-CD5, CD22 and CD40 mAbs were effectively taken up by B-CLL cells, whereas mAbs against CD19, CD20, CD23 and CD45 were not. This study may form a basis for further research to identify antibodies that may serve as carriers for toxins to treat B-CLL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antigens, CD / immunology
  • Antigens, CD19 / immunology
  • Antigens, CD20 / immunology
  • Antigens, Differentiation, B-Lymphocyte / immunology
  • B-Lymphocytes / physiology*
  • CD40 Antigens / immunology
  • CD5 Antigens / immunology
  • Cell Adhesion Molecules*
  • Drug Carriers
  • Endocytosis / physiology*
  • Flow Cytometry
  • Humans
  • Lectins / immunology
  • Leukemia, B-Cell / immunology*
  • Leukocyte Common Antigens / immunology
  • Microscopy, Confocal
  • Receptors, IgE / immunology
  • Sialic Acid Binding Ig-like Lectin 2

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, CD19
  • Antigens, CD20
  • Antigens, Differentiation, B-Lymphocyte
  • CD22 protein, human
  • CD40 Antigens
  • CD5 Antigens
  • Cell Adhesion Molecules
  • Drug Carriers
  • Lectins
  • Receptors, IgE
  • Sialic Acid Binding Ig-like Lectin 2
  • Leukocyte Common Antigens