Abstract
A wheat ethylene receptor homologue (W-er1) was isolated from a wheat stem cDNA library using the Arabidopsis ETR1 cDNA as a probe. The predicted amino acid sequence of W-er1 is over 70% similar to ERS1 from Arabidopsis and exhibits homology to bacterial two-component response regulators within the histidine kinase domain. Northern hybridization demonstrated that W-er1 was expressed in stem, leaf and root tissues. Treatments known to induce senescence of detached leaves including jasmonate, abscisic acid and wounding, increased the accumulation of W-er1 mRNA, while benzyladenine treatment did not. These data suggest that W-er1 may play a role in the process of leaf senescence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology
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Adenine / analogs & derivatives*
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Adenine / pharmacology
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Amino Acid Sequence
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Arabidopsis Proteins
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Benzyl Compounds
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Blotting, Northern
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Chlorophyll / metabolism
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Cyclopentanes / pharmacology
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Plant / drug effects
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Kinetin
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Molecular Sequence Data
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Oxylipins
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Plant Leaves / drug effects
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Plant Leaves / genetics*
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Plant Leaves / growth & development
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Plant Proteins / genetics*
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Purines
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Cell Surface / genetics*
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Sequence Analysis, DNA
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Stress, Mechanical
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Triticum / drug effects
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Triticum / genetics*
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Triticum / metabolism
Substances
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Arabidopsis Proteins
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Benzyl Compounds
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Cyclopentanes
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DNA, Complementary
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ERS protein, Arabidopsis
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Oxylipins
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Plant Proteins
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Purines
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RNA, Messenger
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Receptors, Cell Surface
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Chlorophyll
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jasmonic acid
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Abscisic Acid
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Adenine
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benzylaminopurine
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Kinetin