Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection

Biosens Bioelectron. 2003 May;18(5-6):547-53. doi: 10.1016/s0956-5663(03)00026-5.

Abstract

A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)(20)Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg(2+), Cd(2+), Pb(2+), Cu(2+) and Zn(2+) ions in concentration range of 100 fM-10 mM, and the order of sensitivity was S(Zn)>S(Cu)>S(Hg)>>S(Cd) congruent with S(Pb). The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Validation Study

MeSH terms

  • Biosensing Techniques*
  • Electric Capacitance
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Equipment Design
  • Equipment Failure Analysis*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Glutathione
  • Metalloproteins / biosynthesis*
  • Metalloproteins / chemistry*
  • Metalloproteins / genetics
  • Metals, Heavy / analysis*
  • Metals, Heavy / chemistry*
  • Microchemistry / instrumentation
  • Microchemistry / methods
  • Phytochelatins
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Metalloproteins
  • Metals, Heavy
  • Recombinant Fusion Proteins
  • Phytochelatins
  • Glutathione