Abstract
A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)(20)Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg(2+), Cd(2+), Pb(2+), Cu(2+) and Zn(2+) ions in concentration range of 100 fM-10 mM, and the order of sensitivity was S(Zn)>S(Cu)>S(Hg)>>S(Cd) congruent with S(Pb). The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Validation Study
MeSH terms
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Biosensing Techniques*
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Electric Capacitance
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Electrochemistry / instrumentation*
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Electrochemistry / methods
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Equipment Design
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Equipment Failure Analysis*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Glutathione
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Metalloproteins / biosynthesis*
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Metalloproteins / chemistry*
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Metalloproteins / genetics
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Metals, Heavy / analysis*
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Metals, Heavy / chemistry*
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Microchemistry / instrumentation
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Microchemistry / methods
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Phytochelatins
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Metalloproteins
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Metals, Heavy
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Recombinant Fusion Proteins
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Phytochelatins
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Glutathione