First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination

Chem Commun (Camb). 2003 Mar 21:(6):686-7. doi: 10.1039/b300847a.

Abstract

The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl Carrier Protein / metabolism
  • Acyltransferases / chemistry
  • Acyltransferases / genetics
  • Acyltransferases / metabolism*
  • Anthraquinones / chemistry
  • Anthraquinones / metabolism*
  • Bacterial Proteins*
  • Malonyl Coenzyme A / metabolism
  • Polyketide Synthases
  • Spectrometry, Mass, Electrospray Ionization
  • Streptomyces / enzymology

Substances

  • Acyl Carrier Protein
  • Anthraquinones
  • Bacterial Proteins
  • Malonyl Coenzyme A
  • Polyketide Synthases
  • Acyltransferases
  • polyketide beta-ketoacylsynthase
  • actinorhodin