Amplification of cDNA ends using PCR suppression effect and step-out PCR

Methods Mol Biol. 2003:221:41-9. doi: 10.1385/1-59259-359-3:41.
No abstract available

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular / methods*
  • DNA Primers / genetics
  • DNA, Complementary / genetics*
  • DNA, Single-Stranded / chemistry
  • Electrophoresis, Agar Gel
  • Gene Amplification*
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques / methods*
  • Polymerase Chain Reaction / methods*

Substances

  • DNA Primers
  • DNA, Complementary
  • DNA, Single-Stranded