Addiction to smoking was assessed in staff of Kaunas University of Medicine Hospital based on the results of an anonymous questionnaire. A total of 3090 valid questionnaires were collected (56.5% of whole sample). Prevalence of smokers was 13.3% (37.6% men and 9.7% women), former smokers - 6.0% (16.0% men and 3.7% women) and non-smokers - 80.7% (46.4% men and 86.6% women). The 30-39 year old age men and 18-39 year old women groups present the highest prevalence of smoking (48.8% and 14.9%, accordingly). The highest prevalence of tobacco smoke was found among auxiliary personnel men and women (44.9% and 11.3%, accordingly) and the lowest - among doctors (36.4% and 7.6%, accordingly). The received data enable to evaluate smoking prevalence among University Hospital staff and allow smoking control policy measures.